ltl biotin conjugate Search Results


lotus tetragonolobus lectin biotin conjugated  (Vector Laboratories)
96
Vector Laboratories lotus tetragonolobus lectin biotin conjugated
(a) Immunocytochemistry for SIX2 at day 9 of differentiation revealing NPCs. Scale bar: 50 μm. (b) Immunocytochemistry to identify nephron segments in 2D culture on day 21 of the differentiation. Scale bar: 50 μm. (c) Immunohistochemistry to identify nephron segments in 3D culture with frozen sections on day 21 of differentiation. Scale bar: 50 μm. (d) Whole mount staining for nephrons in 3D culture on day 28 (left: high magnification, scale bar: 50 μm) and 21 (right: low magnification, scale bar: 100 μm). The inset on the right shows DAPI (4′,6-diamidino-2-phenylindole) staining. PODXL: podocalyxin (a podocyte marker). LTL: lotus <t>tetragonolobus</t> <t>lectin</t> (a proximal tubule marker). CDH1: cadherin1 (also known as E-cadherin) (a loop of Henle and distal tubule marker). (e) Bright field imaging of an organoid in 3D culture on day 21. Arrows indicate a glomerular structure. Scale bar: 100 μm. (c) and the left panel of (d) were originally published in ref 15.
Lotus Tetragonolobus Lectin Biotin Conjugated, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lotus tetragonolobus lectin biotin conjugated/product/Vector Laboratories
Average 96 stars, based on 1 article reviews
lotus tetragonolobus lectin biotin conjugated - by Bioz Stars, 2026-03
96/100 stars
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biotin conjugated lotus tetragonolobus lectin  (Vector Laboratories)
94
Vector Laboratories biotin conjugated lotus tetragonolobus lectin
(a) Immunocytochemistry for SIX2 at day 9 of differentiation revealing NPCs. Scale bar: 50 μm. (b) Immunocytochemistry to identify nephron segments in 2D culture on day 21 of the differentiation. Scale bar: 50 μm. (c) Immunohistochemistry to identify nephron segments in 3D culture with frozen sections on day 21 of differentiation. Scale bar: 50 μm. (d) Whole mount staining for nephrons in 3D culture on day 28 (left: high magnification, scale bar: 50 μm) and 21 (right: low magnification, scale bar: 100 μm). The inset on the right shows DAPI (4′,6-diamidino-2-phenylindole) staining. PODXL: podocalyxin (a podocyte marker). LTL: lotus <t>tetragonolobus</t> <t>lectin</t> (a proximal tubule marker). CDH1: cadherin1 (also known as E-cadherin) (a loop of Henle and distal tubule marker). (e) Bright field imaging of an organoid in 3D culture on day 21. Arrows indicate a glomerular structure. Scale bar: 100 μm. (c) and the left panel of (d) were originally published in ref 15.
Biotin Conjugated Lotus Tetragonolobus Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotin conjugated lotus tetragonolobus lectin/product/Vector Laboratories
Average 94 stars, based on 1 article reviews
biotin conjugated lotus tetragonolobus lectin - by Bioz Stars, 2026-03
94/100 stars
  Buy from Supplier
biotin conjugated ltl primary antibody  (Vector Laboratories)
96
Vector Laboratories biotin conjugated ltl primary antibody
(a) Immunocytochemistry for SIX2 at day 9 of differentiation revealing NPCs. Scale bar: 50 μm. (b) Immunocytochemistry to identify nephron segments in 2D culture on day 21 of the differentiation. Scale bar: 50 μm. (c) Immunohistochemistry to identify nephron segments in 3D culture with frozen sections on day 21 of differentiation. Scale bar: 50 μm. (d) Whole mount staining for nephrons in 3D culture on day 28 (left: high magnification, scale bar: 50 μm) and 21 (right: low magnification, scale bar: 100 μm). The inset on the right shows DAPI (4′,6-diamidino-2-phenylindole) staining. PODXL: podocalyxin (a podocyte marker). LTL: lotus <t>tetragonolobus</t> <t>lectin</t> (a proximal tubule marker). CDH1: cadherin1 (also known as E-cadherin) (a loop of Henle and distal tubule marker). (e) Bright field imaging of an organoid in 3D culture on day 21. Arrows indicate a glomerular structure. Scale bar: 100 μm. (c) and the left panel of (d) were originally published in ref 15.
Biotin Conjugated Ltl Primary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotin conjugated ltl primary antibody/product/Vector Laboratories
Average 96 stars, based on 1 article reviews
biotin conjugated ltl primary antibody - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier

Image Search Results


(a) Immunocytochemistry for SIX2 at day 9 of differentiation revealing NPCs. Scale bar: 50 μm. (b) Immunocytochemistry to identify nephron segments in 2D culture on day 21 of the differentiation. Scale bar: 50 μm. (c) Immunohistochemistry to identify nephron segments in 3D culture with frozen sections on day 21 of differentiation. Scale bar: 50 μm. (d) Whole mount staining for nephrons in 3D culture on day 28 (left: high magnification, scale bar: 50 μm) and 21 (right: low magnification, scale bar: 100 μm). The inset on the right shows DAPI (4′,6-diamidino-2-phenylindole) staining. PODXL: podocalyxin (a podocyte marker). LTL: lotus tetragonolobus lectin (a proximal tubule marker). CDH1: cadherin1 (also known as E-cadherin) (a loop of Henle and distal tubule marker). (e) Bright field imaging of an organoid in 3D culture on day 21. Arrows indicate a glomerular structure. Scale bar: 100 μm. (c) and the left panel of (d) were originally published in ref 15.

Journal: Nature protocols

Article Title: Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells

doi: 10.1038/nprot.2016.170

Figure Lengend Snippet: (a) Immunocytochemistry for SIX2 at day 9 of differentiation revealing NPCs. Scale bar: 50 μm. (b) Immunocytochemistry to identify nephron segments in 2D culture on day 21 of the differentiation. Scale bar: 50 μm. (c) Immunohistochemistry to identify nephron segments in 3D culture with frozen sections on day 21 of differentiation. Scale bar: 50 μm. (d) Whole mount staining for nephrons in 3D culture on day 28 (left: high magnification, scale bar: 50 μm) and 21 (right: low magnification, scale bar: 100 μm). The inset on the right shows DAPI (4′,6-diamidino-2-phenylindole) staining. PODXL: podocalyxin (a podocyte marker). LTL: lotus tetragonolobus lectin (a proximal tubule marker). CDH1: cadherin1 (also known as E-cadherin) (a loop of Henle and distal tubule marker). (e) Bright field imaging of an organoid in 3D culture on day 21. Arrows indicate a glomerular structure. Scale bar: 100 μm. (c) and the left panel of (d) were originally published in ref 15.

Article Snippet: Incubate the plate overnight at 4 °C. table ft1 table-wrap mode="anchored" t5 caption a7 Antigen Host Supplier Cat. No. Dilution α-SMA Mouse Sigma F3777 1:500 Aquaporin2 Rabbit Millipore AB3274 1:100 E-cadherin (CDH1) Rat Abcam ab11512 1:500 Endomucin Rat Abcam ab45771 1:500 HOXD11 Mouse Sigma SAB1403944 1:500 KIM1 Goat R&D AF1750 1:500 Laminin Rabbit Sigma L9393 1:500 LHX1 Mouse Developmental Studies Hybridoma Bank 4F2-c 1:50 LTL (lotus tetragonolobus lectin) Biotin conjugated Vector lab B-1325 1:200 PAX8 Rabbit Proteintech 10336-1-AP 1:500 PDGFR-b Goat Novus AF385 1:100 Podocalyxin (PODXL) Goat R&D systems AF1658 1:500 SIX2 Rabbit Proteintech 11562-1-AP 1:500 Uromodulin Rabbit Biomedical Technologies BT-590 1:150 WT-1 Rabbit Santa cruz sc-192 1:50 Open in a separate window Antibody list CRITICAL STEP If the surface of the wells is not covered fully by the antibody dilution buffer, increase the antibody solution amount from 150 to 200 μl.

Techniques: Immunocytochemistry, Immunohistochemistry, Staining, Marker, Imaging

Immunohistochemical staining for CDH1 (cadherin1), KIM1 (kidney injury molecule1), and LTL (lotus tetragonolobus lectin) in kidney organoids after 24 hours treatment with cisplatin 5 μM. LTL+ tubules expressed KIM1 after the treatment, which is a marker for proximal tubular injury. Kidney organoids generated in 3D culture were treated with cisplatin 5 μM for 24 hours from day 23 to 24 of the differentiation. Organoids were fixed and analyzed on day 24. Scale bars: 50 μm. The scale bars are representative of the corresponding right panels.

Journal: Nature protocols

Article Title: Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells

doi: 10.1038/nprot.2016.170

Figure Lengend Snippet: Immunohistochemical staining for CDH1 (cadherin1), KIM1 (kidney injury molecule1), and LTL (lotus tetragonolobus lectin) in kidney organoids after 24 hours treatment with cisplatin 5 μM. LTL+ tubules expressed KIM1 after the treatment, which is a marker for proximal tubular injury. Kidney organoids generated in 3D culture were treated with cisplatin 5 μM for 24 hours from day 23 to 24 of the differentiation. Organoids were fixed and analyzed on day 24. Scale bars: 50 μm. The scale bars are representative of the corresponding right panels.

Article Snippet: Incubate the plate overnight at 4 °C. table ft1 table-wrap mode="anchored" t5 caption a7 Antigen Host Supplier Cat. No. Dilution α-SMA Mouse Sigma F3777 1:500 Aquaporin2 Rabbit Millipore AB3274 1:100 E-cadherin (CDH1) Rat Abcam ab11512 1:500 Endomucin Rat Abcam ab45771 1:500 HOXD11 Mouse Sigma SAB1403944 1:500 KIM1 Goat R&D AF1750 1:500 Laminin Rabbit Sigma L9393 1:500 LHX1 Mouse Developmental Studies Hybridoma Bank 4F2-c 1:50 LTL (lotus tetragonolobus lectin) Biotin conjugated Vector lab B-1325 1:200 PAX8 Rabbit Proteintech 10336-1-AP 1:500 PDGFR-b Goat Novus AF385 1:100 Podocalyxin (PODXL) Goat R&D systems AF1658 1:500 SIX2 Rabbit Proteintech 11562-1-AP 1:500 Uromodulin Rabbit Biomedical Technologies BT-590 1:150 WT-1 Rabbit Santa cruz sc-192 1:50 Open in a separate window Antibody list CRITICAL STEP If the surface of the wells is not covered fully by the antibody dilution buffer, increase the antibody solution amount from 150 to 200 μl.

Techniques: Immunohistochemical staining, Staining, Marker, Generated

Antibody list

Journal: Nature protocols

Article Title: Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells

doi: 10.1038/nprot.2016.170

Figure Lengend Snippet: Antibody list

Article Snippet: Incubate the plate overnight at 4 °C. table ft1 table-wrap mode="anchored" t5 caption a7 Antigen Host Supplier Cat. No. Dilution α-SMA Mouse Sigma F3777 1:500 Aquaporin2 Rabbit Millipore AB3274 1:100 E-cadherin (CDH1) Rat Abcam ab11512 1:500 Endomucin Rat Abcam ab45771 1:500 HOXD11 Mouse Sigma SAB1403944 1:500 KIM1 Goat R&D AF1750 1:500 Laminin Rabbit Sigma L9393 1:500 LHX1 Mouse Developmental Studies Hybridoma Bank 4F2-c 1:50 LTL (lotus tetragonolobus lectin) Biotin conjugated Vector lab B-1325 1:200 PAX8 Rabbit Proteintech 10336-1-AP 1:500 PDGFR-b Goat Novus AF385 1:100 Podocalyxin (PODXL) Goat R&D systems AF1658 1:500 SIX2 Rabbit Proteintech 11562-1-AP 1:500 Uromodulin Rabbit Biomedical Technologies BT-590 1:150 WT-1 Rabbit Santa cruz sc-192 1:50 Open in a separate window Antibody list CRITICAL STEP If the surface of the wells is not covered fully by the antibody dilution buffer, increase the antibody solution amount from 150 to 200 μl.

Techniques: Plasmid Preparation